Fig. 4. MSP analysis is performed to evaluate the methylation status (A, B). ChIP assay is used to assess the relationship of EZH2 and the DR4 promoter in LNCap-LN3 and DU 145 cells (C). After docetaxel or DZNep treatment, the relationship of EZH2 and the promoter lesion of DR4 or DR5 is evaluated (D). ChIP: chromatin immunoprecipitation, DR4: death receptor 4, DR5: death receptor 5, EZH2: enhancer of zeste homolog 2, IgG: immunoglobulin G, MSP: methylation-specific polymerase chain reaction, ns: not significant, PCR: polymerase chain reaction. ^*p<0.05, ^**p<0.01.