Fig. 2. PomX forms filaments that are bundled by PEG8000 in vitro.

a Domain architecture of PomX. PomX domain truncations as used in ref. ³⁴ and here are indicated by vertical lines at the corresponding amino acid position. b Schematic of the experimental setup. c Turbidity measurements of PomX for increasing protein and PEG8000 concentrations. The heat map displays average absorption at 340 nm for n = 3 replicates. d PomX forms filaments that are increasingly bundled with increasing PEG8000 concentrations. Representative maximum intensity projections of confocal z-stacks (left) and high-resolution images (right) of 4 µM PomX-A488 in the presence of increasing concentrations of PEG8000. The experiment was performed three times. Scale bars, 50 µm. e Representative TEM images of PomX in the presence and absence of 4% PEG8000. Frames indicate regions in the zoomed images. Experiments were performed three times. Scale bars, 0.5 µm. f Schematic representation of the bundling effect of PEG8000 on PomX filaments.