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. 2023 Jun 28;6:676. doi: 10.1038/s42003-023-05057-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Confocal immunofluorescence microscopic images of Spd in control and UUO kidneys. Green, anti-Spd antibody; blue, DAPI. Scale bars, 50 µm. b Quantification of the Spd-positive area in control and UUO kidneys (n = 6 in each group). Data are indicated as means ± SD. c Western blot analysis of ARG2 protein levels in the whole kidney of sham and UUO mice. d Relative levels of ARG2 protein normalized to GAPDH in sham and UUO kidneys are shown (n = 6 in each group). Data are indicated as means ± SD. e Immunohistochemistry of ARG2 in control and UUO kidneys. Green, anti-ARG2 antibody; blue, DAPI. Scale bars, 50 µm. White arrowheads indicate ARG2-positive tubules. f Quantification of the ARG2-positive area in sham and UUO kidneys (n = 6 in each group). Data are indicated as means ± SD. *P < 0.05, **P < 0.01. g Immunostaining of Spd in the human kidney. Control, donor kidneys; T0–T2, interstitial fibrosis/tubular atrophy scores from the Oxford classification of IgA nephropathy. The percentages of lesions in the cortical area were as follows: T0 = 0%–25%, T1 = 26%–50%, and T2 = > 50%. Scale bars, 50 µm. h Quantification of the Spd-positive area in human kidneys (n = 6 in each group). Data are indicated as means ± SD. **P < 0.01. Spd spermidine, UUO unilateral ureteral obstruction, ARG2 arginase 2, DAPI 4′,6-diamidino-2-phenylindole, GAPDH glyceraldehyde-3-phosphate dehydrogenase.