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. 2023 Jun 28;6:676. doi: 10.1038/s42003-023-05057-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Confocal immunofluorescence images of Spd in the UUO kidney of WT and Arg2 KO mice. Green, anti-Spd antibody; blue, DAPI. Scale bars, 20 µm. b Quantification of the Spd-positive area (n = 6 in each group). Data are indicated as means ± SD. c Representative images of Sirius red staining in the UUO kidney of WT and Arg2 KO mice. Scale bars, 100 µm. d Quantification of the Sirius red staining-positive area (n = 6 in each group). Data are indicated as means ± SD. e Representative images of Masson trichrome staining in the UUO kidney of WT and Arg2 KO mice. Scale bars, 100 µm. f Quantification of the Masson trichrome staining-positive area (n = 6 in each group). Data are indicated as means ± SD. g Western blot analysis of collagen type 1 protein in the UUO kidney of WT and Arg2 KO mice. Quantification of relative levels of collagen type 1 protein normalized to αβ tubulin are shown on the right (n = 6 in each group). Data are indicated as means ± SD. h Western blot analysis of αSMA protein in the UUO kidney of WT and Arg2 KO mice. Quantification of relative levels of αSMA protein normalized to αβ tubulin are shown on the right (n = 6 in each group). Data are indicated as means ± SD. i Western blot analysis of mature TGFβ protein in the UUO kidney of WT and Arg2 KO mice. Quantification of relative levels of mature TGFβ protein normalized to αβ tubulin are shown on the right (n = 6 in each group). Data are indicated as means ± SD. j Western blot analysis of Nrf2 protein in the UUO kidney of WT and Arg2 KO mice. k Quantification of relative levels of Nrf2 protein normalized to αβ tubulin (n = 6 in each group). Data are indicated as means ± SD. l HO-1 mRNA expression in the UUO kidney of WT and Arg2 KO mice determined by real-time PCR (n = 6 in each group). Data are indicated as means ± SD. m Representative images of Sirius red staining in the UUO kidney of WT and Arg2 KO mice treated with Spd. Scale, 100 µm. n Quantification of the Sirius red staining-positive area (n = 5 in each group). Data are indicated as means ± SD. *P < 0.05, **P < 0.01. NS not significant, UUO unilateral ureteral obstruction, Col1 collagen 1, Spd spermidine, DAPI 4′,6-diamidino-2-phenylindole, αSMA α smooth muscle actin, TGFβ transforming growth factor β1, Nrf2 nuclear factor erythroid 2-related factor 2.