Fig. 3. Overexpression of miR-28a-5p significantly abolishes GAS5 overexpression-mediated suppression of hepatic steatosis, inflammation, and insulin resistance in Ob/Ob mice.

C57BL/6 and Ob/Ob mice were fed a chow diet. When they were 10 weeks old, they were injected with adenoviruses every 10 days. After 3 weeks of the first injection, the mice were used for further analysis. A Food intake was measured every day during ND or HFD feeding (n = 5/group). B The body weight of mice was measured after 3 weeks of the first injection of adenoviruses (n = 5/group). C Liver weights and LW/BW ratios of C57BL/6 or Ob/Ob mice after 3 weeks of the first injection (n = 5/group). D TG, NEFA, and TC levels were measured using ELISA in the livers of adenovirus-injected C57BL/6 and Ob/Ob mice (n = 5/group). E, F H&E and Oil Red O staining of liver sections from adenovirus-injected C57BL/6 and Ob/Ob mice (n = 5/group); Scale bar, 50 µM. G The mRNA expression of lipid metabolism-related genes in the livers of C57BL/6 or Ob/Ob mice was measured using RT‒qPCR analysis (n = 5/group). H The mRNA levels of inflammation-related genes were analysed by RT‒qPCR (n = 5/group). I Serum AST, ALT, and ALP contents were analysed using ELISA in mice (n = 5/group). J Fasting blood glucose levels of C57BL/6 and Ob/Ob mice (n = 5/group). K, L Fasting insulin levels and HOMA-IR scores of adenovirus-injected C57BL/6 and Ob/Ob mice (n = 5/group). M–P GTT and ITT of C57BL/6 and Ob/Ob mice after 3 weeks of adenovirus injection and the areas under the curve (AUCs) for the GTT and ITT (N = 5/group). Q RT‒qPCR analysis of PEPCK and G6Pase expression in the livers of mice (N = 5/group). The data are presented as the mean ± S.D. of three independent experiments. A p value of <0.05 was considered statistically significant; and *p < 0.05, **p < 0.01, ***p < 0.001 assessed via a ANOVA with the Bonferroni post hoc test for comparisons among more than two groups (A–Q); ns not significant.