Skip to main content
. 2023 May 12;30(7):1679–1694. doi: 10.1038/s41418-023-01171-8

Fig. 7. CircRILPL1 promotes YAP nuclear translocation through mediating its binding to IPO7.

Fig. 7

A The binding between circRILPL1 and IPO7 protein was detected in NPC cells by RNA pull-down with biotin-labeled circRILPL1 probe. GAPDH was used as a negative control. B The binding between circRILPL1 and IPO7 protein was examined in NPC cells using anti-IPO7 antibody by RIP assay. The fold enrichment of circRILPL1 is relative to that of the control IgG. C CircRILPL1 promotes the nuclear translocation of IPO7 in NPC cells as examined by cytosolic/nuclear protein fractionation. PARP was used as a nuclear marker and β-tubulin as a cytoplasmic marker. D. Co-localization of YAP and IPO7 in NPC cells after overexpression of circRILPL1. DAPI: blue; YAP: red; IPO7: green; Scale bar = 10 μm. E The effect of circRILPL1 on the interaction between YAP and IPO7 was detected by immunoprecipitation using anti-Flag (IPO7) antibody, followed by western blotting using YAP antibody in NPC cells transfected with Flag-IPO7 plasmid, co-transfected with circRILPL1 overexpression plasmid or empty vector. GAPDH was used as a negative control. F The transcriptional activity of YAP was measured by luciferase reporter assay after overexpression or knockdown of circRILPL1 or IPO7 in NPC cells. G Knockdown of IPO7 blocked the nuclear import of YAP induced by circRILPL1 in NPC cells. PARP was used as a nuclear marker and β-tubulin as a cytoplasmic marker. H The effects of knockdown of IPO7 or ROCK1 on circRILPL1-induced YAP nuclear translocation in HNE2 cells. Data were presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.