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. 2023 May 12;30(7):1679–1694. doi: 10.1038/s41418-023-01171-8

Fig. 8. CircRILPL1-YAP signaling promotes the transcription of CAPN2 and PXN.

Fig. 8

A The expression levels of CAPN2 and PXN mRNA were examined by qRT-PCR in NPC cells after overexpression or knockdown of circRILPL1. B The expressions of CAPN2 and PXN protein were examined by western blotting in NPC cells after overexpression or knockdown of circRILPL1. C The effects of circRILPL1 and YAP on the expressions of CAPN2 and PXN mRNA in NPC cells were detected by qRT-PCR. D The effect of circRILPL1 on the enrichment of YAP on CAPN2 and PXN promoters was examined by ChIP assay. E Schematic diagram of the signaling pathways regulated by circRILPL1 in NPC cells. CircRILPL1 inhibits the LATS1 kinase through binding to and activating ROCK1, thereby suppressing the phosphorylation of YAP. In cooperation with the nuclear transport receptor IPO7, circRILPL1 prompts loading of YAP onto IPO7 and thus enhances YAP translocation into nucleus, where YAP activates the transcription of cytoskeleton remodeling genes CAPN2 and PXN. CircRILPL1 activates the Hippo-YAP signaling pathway, ultimately resulting in biomechanical differences, proliferation, and metastasis of NPC cells. Data were presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.