Figure 1.

Timeline of the experiment. On day 0, an HI insult was performed after surgical preparation and stabilization. The HI insult was followed by rescue with 100% O₂. After the rescue, the piglets were randomized into NT, H₂, TH, and H₂-TH groups and the treatment was given accordingly for 24 h. In the TH and H₂-TH groups, rewarming was performed. From day 1 to day 5, the piglets were allowed to recover, nursed, and fed. Neurological function was observed every 6 h from day 1 to day 5 after the insult. On day 5, the piglets were euthanized and their brains were harvested for histological analysis.