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. 2023 Jun 7;30(7):1786–1798. doi: 10.1038/s41418-023-01179-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a mRNA-seq analysis was performed in cardiomyocytes infected with or without adenovirus harboring ATF5 (Ad-ATF5 or Ad-Con). b qPCR validation of the expression levels of genes which were selected from the results of mRNA-seq data in Ad-ATF5 or Ad-Con treated cardiomyocytes (n = 5 independent experiments). c Cardiomyocytes were transfected with ATF5-siRNA (si-ATF5) and its control (si-NC). Inca1 mRNA and protein levels were evaluated by qRT-PCR and western blot (n = 5 independent experiments). d Motif analysis of the ATF5 bound regions. e Isolated neonatal cardiomyocytes were infected with adenovirus harboring ATF5 (Ad-ATF5) or its control (Ad-Con) for 24 h. CHIP-qPCR assay was performed using antibodies against ATF5 or IgG (n = 5 independent experiments). f The mRNA (left panel) and protein (right panel) levels of INCA1 in TMEM11 KO and WT mice hearts (n = 6 mice per group). g The mRNA (left panel) and protein (right panel) levels of INCA1 in TMEM11 Tg and WT mice hearts (n = 6 mice per group). h Neonatal cardiomyocytes were infected with adenovirus harboring TMEM11, and transfected with si-NC or si-ATF5 for 48 h. The mRNA (left panel) and protein (right panel) levels of Inca1 were analyzed (n = 5 independent experiments). Data are presented as the mean ± s.d. One-way ANOVA (h), two-sided Student’s t test (b, c, f and g) or two-way ANOVA (e) was performed.