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. 2023 Jun 28;13(7):e1318. doi: 10.1002/ctm2.1318

FIGURE 2.

FIGURE 2

CircAFF2 was identified with circular features. (A) hsa_circ_0001947 is formed by the circularisation of exon 3 of AFF2 gene with a spliced length of 861 bp, which was verified by Sanger sequencing. (B) cDNA and genomic gDNA reverse transcribed from circAFF2 were subjected to PCR amplification in HT‐29 and HCT‐166 cells. (C) Differences between circAFF2 and linear RNA upon RNase R treatment in HT‐29 and HCT‐116 cells. (D) Nucleocytoplasmic separation assay to characterise the distribution of circAFF2 in HT‐29 and HCT‐116 cells. (E) The distribution of circAFF2 was examined by fluorescence in situ hybridisation (FISH) assay in HT‐29 and HCT‐116 cells. *** p < .001, ** p < .01, * p < .05.