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. 2023 Jun 28;13(7):e1318. doi: 10.1002/ctm2.1318

FIGURE 5.

FIGURE 5

ALKBH5/YTHDF2 regulates colorectal cancer (CRC) radiosensitivity through circAFF2 in vitro. (A) Colony formation assay to detect the colony formation number of cells after 4 Gy irradiation in HT‐29 and HCT‐116 cells transfected with Len‐sh‐NC, Len‐sh‐ALKBH5, Len‐sh‐ALKBH5 + Len‐OE‐circAFF2, Len‐sh‐YTHDF2 and Len‐sh‐YTHDF2 + Len‐sh‐circAFF2. Western blot (B) and ELISA assay (C) to determine γ‐H2AX expression 6 h after 4 Gy irradiation in HT‐29 and HCT‐116 cells transfected with Len‐sh‐NC, Len‐sh‐ALKBH5, Len‐sh‐ALKBH5 + Len‐OE‐circAFF2, Len‐sh‐YTHDF2 and Len‐sh‐YTHDF2 + Len‐sh‐circAFF2. (D) One day after 4 Gy irradiation, apoptosis was determined in HT‐29 and HCT‐116 cells transfected with Len‐sh‐NC, Len‐sh‐ALKBH5, Len‐sh‐ALKBH5 + Len‐OE‐circAFF2, Len‐sh‐YTHDF2 and Len‐sh‐YTHDF2 + Len‐sh‐circAFF2 by flow cytometry. *** p < .001, ** p < .01, * p < .05.