Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 28;13(7):e1318. doi: 10.1002/ctm2.1318

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

CircAFF2 inhibits Cullin neddylation by binding to CAND1. (A) RNA pull‐down and liquid chromatography–mass spectrometry (LC–MS) were used to detect possible circAFF2 binding proteins. (B) RNA immunoprecipitation (RIP) validates the binding of CAND1 with circAFF2. (C) Neddylation assay to detect Cullin‐neddylation level in circAFF2‐overexpressed or circAFF2‐depleted HT‐29 and HCT‐cells before and after irradiation. (D) Co‐immunoprecipitation (co‐IP) to detect binding levels of Cullin1–CAND1 in circAFF2‐overexpressed or circAFF2‐depleted HT‐29 and HCT‐116 cells before and after irradiation. ^*** p < .001, ^** p < .01, ^* p < .05.