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. 2023 Jun 28;13(7):e1318. doi: 10.1002/ctm2.1318

FIGURE 8.

FIGURE 8

CircAFF2 regulates the radiosensitivity of colorectal cancer (CRC) by binding to CAND1. (A) Neddylation assay to detect Cullin‐neddylation level in HT‐29 and HCT‐116 cells transfected with Len‐OE‐NC, Len‐OE‐circAFF2, Len‐OE‐NC + siCAND and Len‐OE‐circAFF2 + siCAND1. (B) Colony formation assay to detect the colony formation number of cells after 4 Gy irradiation in HT‐29 and HCT‐116 cells transfected with Len‐OE‐NC, Len‐OE‐circAFF2, Len‐OE‐NC + siCAND and Len‐OE‐circAFF2 + siCAND1. Western blot (C) and ELISA assay (D) to determine the γ‐H2AX expression in HT‐29 and HCT‐116 cells transfected with Len‐OE‐NC, Len‐OE‐circAFF2, Len‐OE‐NC + siCAND and Len‐OE‐circAFF2 + siCAND1. (E) Flow cytometry to detect the apoptosis of HT‐29 and HCT‐116 cells transfected with Len‐OE‐NC, Len‐OE‐circAFF2, Len‐OE‐NC + siCAND and Len‐OE‐circAFF2 + siCAND1 1 day after 4 Gy irradiation. *** p < .001, ** p < .01, * p < .05.