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Calculated 3D cell and nuclear shapes compared to data of primary human umbilical vein endothelial cells spread on patterned 1600-μm2 rectangles of varying aspect ratio, as reported by Versaeval et al. Nat Comm 2012 (Versaevel et al., 2012). Calculated x-z nuclear profiles and nuclear heights closely agree with the experimental nuclear shapes, with similarly increasing nuclear lengths with aspect ratios of (A) 2:1, (B) 4:1, and (C) 10:1. Scale bars are 10 microns.
