figs1.

Construction of the controlled random gene perturbation system. Antisense transcription initiated at TRE leads to loss of target gene function and is regulated by doxycycline (A). Sense transcription initiated at TRE lead to gain of target gene function (B). Doxycycline regulated gene expression in candidate clones. Gene expression was measured by luciferase reporter activities in the presence or absence of doxycycline (C). Titration of the transfection efficiency of the gene search vector (D). Titration of the gene search vector and transposase for co-transfection (E). Representative G-418 clones after transfected with the gene search vector, with or without transposase (F).