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. 2022 Jul 7;10(3):687–689. doi: 10.1016/j.gendis.2022.06.006

Figure 1.

Fig. 1

Identification of ARPC1B gene as a key regulator in cancer metastasis. (A) Flow chart of the experimental approach. (B) Genes identified from lung metastasized G418 resistant clones by Splinkerette PCR in three rounds of screening. (C) Decreased levels of ARPC1B in clone M45 and its negative regulation by doxycycline (DOX). This regulation was absent in parent clone 2D2. (D, E) Lung metastasis of clone M45 was inhibited by doxycycline. Representative gross lung pathology and histological staining are shown (D). The quantification of mice with metastatic lung tumor in the presence or absence of doxycycline is shown (E). (F) The gene tree of ARPC1B was constructed using ENSEMBLE, genetic evolutionary tree analysis identifying gene bifurcation resulted in ARPC1A and ARPC1B in mammals. (G) Silencing of ARPC1B increased the expression of ARPC1A and vice versa in pancreatic cancer cell line ASPC-1. (H, I)In vivo experiments confirmed that the overexpression of ARPC1A and the low expression of ARPC1B could significantly increase the number of pulmonary metastases (H). The quantification of mice with metastatic lung tumor in ORF-ARPC1A group, sh-ARPC1B group and ORF-ARPC1A + sh-ARPC1B (I).