Table 1. List of primers and probes used for molecular analysis.
| Reagent | Oligo_Name | Sequence (5' - 3') | Assay description
Green: FAM Yellow: HEX Red: Atto647N |
Optimized Concentration (nM) | |
|---|---|---|---|---|---|
| Primer | kdr_F | CATTTTTCTTGGCCACTGTAGTGAT | kdr_Vgsc-L995F/S | none | 500 |
| Primer | kdr_R | CGATCTTGGTCCATGTTAATTTGCA | kdr_Vgsc-L995F/S | none | 200 |
| Probe | kdr-wt_P | CTTACGACTAAATTTC | wild type - kdr_Vgsc-L995 | HEX | 500 |
| Probe | kdrW-mt_P | ACGACAAAATTTC | mutant - kdr-west_Vgsc-995F | FAM | 500 |
| Probe | kdrE-mt_P | ACGACTGAATTTC | mutant - kdr-east_Vgsc-995S | Atto647N | 500 |
| Primer | RPS7_Fj | CCACCATCGAACACAAAGTTGA | RPS7-Detox (A-D) | none | 100 |
| Primer | RPS7_R | TGCTGCAAACTTCGGCTATTC | RPS7-Detox (A-D) | none | 200 |
| Probe | RPS7_P1 | CCGTGACGTTACGTTCGAATTCCCA | RPS7-Detox (A-D) | FAM | 250 |
| Primer | P3_Fj | ACAATGTGATTGACGAAACCCT | CYP6P3-Detox (A) | none | 400 |
| Primer | P3_R | GGATCACATGCTTTGTGCCG | CYP6P3-Detox (A) | none | 500 |
| Probe | P3_P1 | ACCCGCGTACCGTCTGTGGACT | CYP6P3-Detox (A) | HEX | 350 |
| Primer | M2_F2 | CTGGCGTTGAATCCAGAGGT | CYP6M2-Detox (A) | none | 600 |
| Primer | M2_Rj | GATACTTGCGCAGTGATTCATTAAG | CYP6M2-Detox (A) | none | 400 |
| Probe | M2_P | AGAGAAATCCTGCAAAAGCACAACGGAGA | CYP6M2-Detox (A) | Atto647N | 250 |
| Primer | K1_F | CCGACACGTGGTGATGGATAC | CYP9K1-Detox (B) | none | 200 |
| Primer | K1_Rj | CGTCGTCGGTCCAGTCAAC | CYP9K1-Detox (B) | none | 400 |
| Probe | K1_P | CAATCTTCTGATGCAGGCCCGCAA | CYP9K1-Detox (B) | HEX | 300 |
| Primer | P4_Fj | CTGGACAACGTTATCAATGAAACC | CYP6P4-Detox (B) | none | 400 |
| Primer | P4_R | GCACGGTGTAATCACGCATC | CYP6P4-Detox (B) | none | 500 |
| Probe | P4_P | CCGATCGAGTCACTTTCGCGCG | CYP6P4-Detox (B) | Atto647N | 300 |
| Primer | Z1_Fj | CCCGCAACTGTATCGGTCTG | CYP6Z1-Detox (C) | none | 100 |
| Primer | Z1_R | TTCGGTGCCAGTGTGATTGA | CYP6Z1-Detox (C) | none | 600 |
| Probe | Z1_P1 | TGATGCTGTCCCGATTTAACTTTTCGGC | CYP6Z1-Detox (C) | HEX | 250 |
| Primer | TE2_Fj | CCGGAATTTGTGAAGCTAAACC | GSTE2-Detox (C) | none | 100 |
| Primer | TE2_R | GCTTGACGGGGTCTTTCGG | GSTE2-Detox (C) | none | 400 |
| Probe | TE2_P | CGGTACGATCATCACCGAGAGCCAC | GSTE2-Detox (C) | Atto647N | 300 |
| Primer | P1_Fj | ACAGGTGGTGAACGAAACCC | CYP6P1-Detox (D) | none | 100 |
| Primer | P1_R | GGTGTAATCCTGTCCCGCAA | CYP6P1-Detox (D) | none | 500 |
| Probe | P1_P | CCGCTCGAAACGACGCTGCG | CYP6P1-Detox (D) | HEX | 300 |
| Primer | G16_Fj | GTCCAAGAAGTTGCGTCGGAC | CYP4G16-Detox (D) | none | 200 |
| Primer | G16_R | TCTTCGATTTGCGTTGACGTG | CYP4G16-Detox (D) | none | 200 |
| Probe | G16_P1 | CTGCAGGCCGACATCATTTTGAAGC | CYP4G16-Detox (D) | Atto647N | 300 |