TABLE 2.

Primers used for quantitative RT-PCR.

Gene	Forward primer	Reverse primer	PCR product (bp)	T a
Pgc-1α	ACTATGAATCAAGCCACTACAGAC	TTCATCCCTCTTGAGCCTTTCG	143	60
Tfam	AAGACCTCGTTCAGCATATAACATT	TTTTCCAAGCCTCATTTACAAGC	104	60
CoxIV	TGGGACTATGACAAGAATGAGTGG	TTAGCATGGACCATTGGATACGG	113	61
CytC	ATAGGGGCATGTCACCTCAAAC	GTGGTTAGCCATGACCTGAAAG	172	60
eNOS	AGCGGCTGGTACATGAGTTC	GATGAGGTTGTCCTGGTGTCC	116	60
Sod1	GGCTTCTCGTCTTGCTCTC	AACTGGTTCACCGCTTGC	153	60
Sod2	GCCTCCCAGACCTGCCTTAC	GTGGTACTTCTCCTCGGTGGCG	131	63
Cat	CACTGACGAGATGGCACACTTTG	TGGAGAACCGAACGGCAATAGG	173	63
Gpx1	TCTGGGACCTCGTGGACTG	CACTTCGCACTTCTCAAACAATG	157	62
Gapdh	TGACGTGCCGCCTGGAGAAA	AGTGTAGCCCAAGATGCCCTTCAG	98	63
mtDNA	ACATGCAAACCTCCATAGACCGG	TCACTGCTGAGTCCCGTGGG	131	62
gDNA	GGTCGCGGTGTGGGCATTTG	CGTGATCGTAGCGTCTGGTT	108	60

Bp, base pairs; T_a, temperature of annealing; Pgc-1α, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Tfam, Transcription Factor A, mitochondrial; CoxIV, cytochrome c oxidase subunit 411; CytC, cytochrome c; eNOS, endothelial nitric oxide synthase; Sod1, superoxide dismutase 1, cytosolic; Sod2, superoxide dismutase 2, mitochondrial; Cat, catalase; Gpx1; Glutathione Peroxidase 1; Gapdh, Glyceraldehyde 3-phosphate dehydrogenase. mtDNA, mitochondrial DNA. gDNA, genomic DNA.