Skip to main content
. 2023 Jun 12;95(25):9520–9530. doi: 10.1021/acs.analchem.3c00772

Table 2. Kinetic Parameters of Interactions between EVs and Anti-CD9, Anti-CD63, and Anti-CD81 Using a Two-State Reaction Model.

  anti-CD9 anti-CD63 anti-CD81
ka1 [M–1·s–1] (1.67 ± 0.05) × 102 (3.48 ± 0.02) × 103 (1.76 ± 0.10) × 102
kd1 [s–1] (6.66 ± 0.12) × 10–4 (9.41 ± 0.11) × 10–1 (1.43 ± 0.09) × 10–2
graphic file with name ac3c00772_m002.jpg
 3.99 × 10–6 2.70 × 10–4 8.13 × 10–5
ka2 [s–1] (7.46 ± 0.07) × 10–2 (6.81 ± 0.11) × 10–2 (1.67 ± 0.09) × 10–1
kd2 [s–1] (1.87 ± 0.03) × 10–6 (1.11 ± 0.05) × 10–5 (1.06 ± 0.07) × 10–2
graphic file with name ac3c00772_m003.jpg
 2.51 × 10–5 1.63 × 10–4 6.35 × 10–2
KD = KD1•KD2 [M] 10.00 × 10–11 4.10 × 10–8 5.16 × 10–6
KDa [M] 9.98 × 10–11 4.41 × 10–8 4.77 × 10–6
a

Value determined by fitting a two-state reaction model.