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. 2023 May 19;42(13):e112504. doi: 10.15252/embj.2022112504

Figure 3. The loop stabilizes end‐on Ndc80‐microtubule interactions under force.

Figure 3

  1. Schematic of the optical trap experiment and a typical force trace. A glass bead coated with full‐length or loopless Ndc80 trimers is held in an optical trap near a microtubule end. The displacement of the bead (left Y axis) and the corresponding force (right Y axis) are shown along and across the microtubules axis (black and gray, respectively). Typical stages of an experiment (steps 1–8) are described.
  2. Correlations of stall duration and stall force. Each datapoint represents a single stall event. Filled squares: stalls resulting in microtubule rescue. Open squares: stalls resulting in bead detachment from the microtubule.
  3. The frequency of rescue events after a stall is plotted for full‐length (n = 46) and loopless (n = 44) Ndc80 after binning based on stall duration.

Source data are available online for this figure.