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. 2023 May 19;42(13):e112504. doi: 10.15252/embj.2022112504

Figure EV4. Loop‐dependent binding between Ndc80 monomers and trimers on microtubules.

Figure EV4

  1. Supplementary information for Fig 5A–D. High‐speed recordings to quantify residence time of wild‐type and loopless Ndc80 monomers. The top two kymographs show a microtubule with monomers binding and unbinding. The lower two kymographs show the binding and unbinding of Ndc80 monomers to a microtubule with a Ndc80 trimer. Since trimers are practically motionless on this timescale, only their initial location was recorded and indicated. Corresponding mean residence times and SEM. are shown. The number of analyzed events is indicated. Scale bar: 5 μm.
  2. Distribution of residence times of wild‐type and loopless Ndc80 monomers associating with microtubule‐bound Ndc80 trimers. A single‐exponential fit described the residence time of Ndc80Δloop, likely corresponding to Ndc80Δloop:microtubule off‐rates. Residence time of full‐length Ndc80 could be fitted with two exponents, likely corresponding to Ndc80:microtubule and Ndc80:trimer:microtubule off‐rates.
  3. Typical kymographs showing Ndc80 trimers (magenta) and transiently binding Ndc80 monomers (yellow). Scale bars: vertical (100 s), horizontal (5 μm).

Source data are available online for this figure.