Figure 1.

M6A levels were reduced in endometriosis and downregulated METTL3 accounted for aberrant m⁶A modification. (A) M⁶A levels in normal control endometrium (NC; n = 50), EU (n = 25), and ectopic endometrium (EC; n = 23). (B) mRNA levels of m⁶A-associated genes in the tissues detected by qRT-PCR. NC (n = 16), EU (n = 11), and EC (n = 11). (C–F) Protein levels of m⁶A-associated genes in the tissues detected by western blot analysis. NC (n = 8), EU (n = 8), and EC (n = 8). (G) Protein levels of METTL3 in the tissues detected by immunohistochemical analysis. NC (n = 7), EU (n = 7), and EC (n = 7). All experiments were repeated in triplicate or quadruplicate. Data with error bars are presented to indicate the mean ± SEM values. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001.