Figure 2.

METTL3 regulated the migration and invasion of HESCs. (A and B) Overexpression and knockdown efficacy of METTL3 at mRNA levels in HESCs (si-METTL3-3 was chosen to perform in subsequent studies as it exhibited the best knockdown efficacy to METTL3). (C and D) Overexpression and knockdown efficacy of METTL3 at protein levels in HESCs. (E) Dot blot assays were used to detect the m⁶A changes upon METTL3 overexpression or knockdown in HESCs. Methylene blue stain was performed as control. (F–H) Transwell assays were used to evaluate the migrating and invasive abilities of HESCs upon METTL3 overexpression or knockdown (scale bar = 100 μm). The number of migrating and invasive cells is presented on the right. All experiments were repeated in triplicate or quadruplicate. Data with error bars are presented to indicate the mean ± SEM values. ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001.