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. 2023 Jun 29;19:63. doi: 10.1186/s13007-023-01022-0

Fig. 2.

Fig. 2

 A rapid bioassay to evaluate plant thermotolerance. (A) Experimental design of heat shock bioassay using hydroponic system. (B) Seedlings germinated directly on the MS liquid medium showed low rates of true leaf development and stopped growth. Instead, seedlings germinated on the MS agar plate then transferred to the MS liquid medium were able to establish well. (C) Linear regression showed high correlation between absorbance at 665 and 648 nm (A665 and A648) measured by the spectrophotometer and microplate reader