FIG 1.

FEZ1 depletion or IFN-β treatment reduces early HIV-1 infection in CHME3 cells. (A) Western blot (WB) analysis showing ISG (MxB, ISG56, and IFITM1) levels in CHME3 cells either treated with the negative control (NC) or FEZ1-specific siRNAs (FEZ1-C) or with various concentrations of IFN-β (low, 4 U; high, 1,000 U, respectively). (B to D) CHME3 cells treated with either NC or FEZ1-C siRNAs (B) or with low (C) or high (D) IFN-β concentrations were infected with HIV-1 carrying a luciferase reporter and pseudotyped with WT envelope (HIV-1-WT-Luc) followed by measurements of luciferase activity. Data are shown as the mean ± SD. Statistical significance was calculated using a t test. **, P ≤ 0.01; ****, P ≤ 0.0001. Results are representative of 3 independent biological repeats except in panel B, where the results are representative of 2 experimental replicates.