FIG 1.

TMEM41B expression is unregulated by PRV infection. (A) Mice were mock infected or intranasally infected with PRV-QXX (5 × 10³ TCID₅₀/50 μL per mouse) for 3 days. The levels of TMEM41B mRNA expression in the kidney, brain, lung, and liver were assessed by qRT-PCR analysis (n = 3 per group). *, P < 0.05; **, P < 0.01; ***, P < 0.001. (B to D) Mice were treated as described in A. TMEM41B and PRV gB in the liver (B), lung (C), and kidney (D) were assessed by immunoblotting analysis (n = 3 per group). (E) PK-15 cells were infected with PRV-QXX (MOI = 1) for 0 to 12 h; h.p.i., hours postinfection. The level of TMEM41B mRNA expression was assessed by qRT-PCR analysis. *, P < 0.05; ***, P < 0.001. (F) PK-15 cells were infected with PRV-QXX (MOI = 1) for 0 to 24 h. TMEM41B and PRV gB were assessed by immunoblotting analysis. (G) PK-15 cells were transfected with the TMEM41B-GFP plasmid for 12 h and then mock infected or infected with PRV-QXX (MOI = 1) for 24 h. The colocalization of TMEM41B-GFP with the ER (calnexin, red) was analyzed by immunofluorescence analysis. Scale bar = 10 μm. (H) Quantification of the colocalization of TMEM41B with calnexin from panel G by ImageJ (n = 15).