Fig 3.

Western blots detecting the sybodies (SB) bound onto the surface of Mmr biofilms after co-incubation with His-myc-tagged GroEL1 (A and B) and GroEL2 (C–F) sybodies. Mmr biofilms were incubated with 0.1, 1, or 5 µg of GroEL1 sybodies or 1 or 5 µg of His-GroEL2 sybodies followed by washes of the biofilm pellets, boiling in sample buffer, SDS-PAGE, and detection of the bound sybodies in the sample by Western blot using anti-His (in GroEL1-SB experiments) or anti-myc (in GroEL2-SB experiments) antibodies. In some of the experiments, the first lane (positive control) shows ~50 ng of the sybody directly loaded onto the gel. The negative control (0 µg) lane contains Mmr biofilm without sybodies.