Fig 4.

GroEL1 and GroEL2 sybodies bind biofilms in vitro as well as Mmr inside granulomas ex vivo in microscopy experiments. DAPI (cyan) was used as a counterstain for DNA. Mycobacterial biofilms were stained with green fluorescence-labeled sybodies (yellow) (D–F) or with myc-tagged sybodies + green fluorescent anti-myc antibodies (yellow) (B and C) or green fluorescent anti-myc antibody only as a negative control (yellow) (A). In the granuloma experiments (G–I front view, J–L side view), bacteria (purple) expressing a red fluorescent protein were used to allow the extraction of the granulomas. Granulomas were incubated with 100 µg/mL of green fluorescence-labeled GroEL-binding sybodies and imaged with confocal microscopy. All scale bars are 10 µm.