TABLE 2.
Characteristics of GroEL1 and GroEL2 sybodies
| Target | Sybody number | Molecular weight (Dalton) | Size exclusion chromatography profile | Approximate KD to purified target (nM) | Binding to Mmr biofilms |
Protein sequence |
|---|---|---|---|---|---|---|
| GroEL1 | 1 | 15,767 | Monomeric | 130 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVEQRQMYWYR QAPGKEREWVAAIQSYGKRTKYADSVKGRFTISRDNAKN TVYLQMNSLKPEDTAVYYCVVYVGGGYKGQGTQVTVSA GRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL1 | 5 | 15,648 | Monomeric | 127 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVEHKQMRWYR QAPGKEREWVAAIESSGQYTIYADSVKGRFTISRDNAKNT VYLQMNSLKPEDTAVYYCFVGVGAGYYGQGTQVTVSAG RAGEQKLISEEDLNSAVDHHHHHH |
| GroEL1 | 9 | 15,522 | Monomeric | 64 | No | QVQLVESGGGLVQAGGSLRLSCAASGLPVWQQGMTWY RQAPGKEREWVAAIDSVGAQTYYADSVKGRFTISRDNAK NTVYLQMNSLKPEDTAVYYCAVNVGARYIGQGTQVTVSA GRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL1 | 12 | 16,539 | Monomeric | 14 | NA a | QVQLVESGGGLVQAGGSLRLSCAASGFPVTQAWMEWY RQAPGKEREWVAAIFSHGGGTFYADSVKGRFTISRDNAK NTVYLQMNSLKPEDTAVYYCNVKDTGERDNWYDYWGQ GTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL1 | 14 | 16,404 | Mostly Monomeric/sticky | 31 | Yes |
QVQLVESGGGLVQAGGSLRLSCAASGFPVXNAYMHWY RQAPGKEREWVAAILSSGAHTLYADSVKGRFTISRDNAK NTVYLQMNSLKPEDTAVYYCNVKDYGAGVRYYDYWG
QGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL1 | 18 | 16,520 | Monomeric | 22 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVKTKHMYWYR QAPGKEREWVAAITSIGMITAYADSVKGRFTISRDNAKNT VYLQMNSLKPEDTAVYYCNVKDWGTNRQAYDYWGQGT QVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL1 | 31 | 17,198 | Monomeric | 92 | Yes |
QVQLVESGGGSVQAGGSLRLSCAASGTIYKIYYLGWFRQ
APGKEREGVAALNTFSGGTYYADSVKGRFTVSLDNAKN TVYLQMNSLKPEDTALYYCAAAYDMEGYAWPLYWWH YEYWGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHH HH |
| GroEL2 | 1 | 15,395 | Monomeric | 591 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVAITYMHWYR QAPGKEREWVAAISSTGKTTWYADSVKGRFTISRDNAKN TVYLQMNSLKPEDTAVYYCLVEVGHYYKGQGTQVTVSAG RAGEQKLISEEDLNSAVDHHHHHH* |
| GroEL2 | 2 | 15,087 | Monomeric | 157 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVSSSTMTWYR QAPGKEREWVAAIDSVGNETYYADSVKGRFTISRDNAKN TVYLQMNSLKPEDTAVYYCAVFVGSYYGQGTQVTVSAGR AGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 3 | 15,554 | Monomeric | 18 | Yes | QVQLVESGGGLVQAGGSLRLSCAASGFPVAYWEMVWY RQAPGKEREWVAAIRSTGWKTVYADSVKGRFTISRDNA KNTVYLQMNSLKPEDTAVYYCTAVYVGVHYKGQGTQV TVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 7 | 16,345 | Monomeric | 100 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVNDAWMYWY RQAPGKEREWVAAIMSMGFGTWYADSVKGRFTISRDNA KNTVYLQMNSLKPEDTAVYYCNVKDRGKEHFSYDYWGQ GTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 8 | 16,234 | Monomeric | 13 | Yes | QVQLVESGGGLVQAGGSLRLSCAASGFPVYMSWMYW YRQAPGKEREWVAAIMSEGAGTWYADSVKGRFTISRD NAKNTVYLQMNSLKPEDTAVYYCNVKDTGSFHAQYDY WGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 9 | 16,333 | Monomeric | 277 | Yes | QVQLVESGGGLVQAGGSLRLSCAASGFPVYQSWMYWY RQAPGKEREWVAAIMSDGSGTWYADSVKGRFTISRDN AKNTVYLQMNSLKPEDTAVYYCNVKDFGHSRSRYDYW GQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 10 | 16,343 | Monomeric | 193 | Yes | QVQLVESGGGLVQAGGSLRLSCAASGFPVKHWYMHW YRQAPGKEREWVAAIQSTGSYTAYADSVKGRFTISRDN AKNTVYLQMNSLKPEDTAVYYCNVKEYGFYHASYDYW GQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 11 | 16,259 | Monomeric | 271 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVDSAYMWWY RQAPGKEREWVAAIESNGEYTFYADSVKGRFTISRDNAKN TVYLQMNSLKPEDTAVYYCNVKDTGAHHSYYDYWGQGT QVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 16 | 16,232 | Monomeric | 15 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVSSSTMTWYR QAPGKEREWVAAIESWGAYTWYADSVKGRFTISRDNAK NTVYLQMNSLKPEDTAVYYCNVKDYDGVADVIYDYWGQ GTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 19 | 16,311 | Monomeric | 366 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVEWLEMAWYR QAPGKEREWVAAIYSYGMETEYADSVKGRFTISRDNAKN TVYLQMNSLKPEDTAVYYCNVKDGGHAAWWYDYWGQ GTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 22 | 16,211 | Monomeric | 507 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVYHSWMYWYR QAPGKEREWVAAIMSDGHGTWYADSVKGRFTISRDNAK NTVYLQMNSLKPEDTAVYYCNVKDTGSSTTIYDYWGQGT QVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 27 | 16,250 | Monomeric | 101 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVWKAYMWWY RQAPGKEREWVAAIESNGAYTFYADSVKGRFTISRDNAK NTVYLQMNSLKPEDTAVYYCNVKDTGSDSENYDYWGQG TQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 28 | 16,134 | Monomeric | 35 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVDAYWMYWY RQAPGKEREWVAAIMSSGHGTWYADSVKGRFTISRDNA KNTVYLQMNSLKPEDTAVYSCNVKDKGAQAAWYDYWG QGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 31 | 16,488 | Monomeric | 66 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVWMEWMYW YRQAPGKEREWVAAIMSEGDGTWYADSVKGRFTISRDN AKNTVYLQMNSLKPEDTAVYYCNVKDFGYNNNYYDYWG QGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 32 | 16,078 | Monomeric | 153 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVVSQFMEWHR QAPGKEREWVAAIDSTGYSTFYADSVKGRFTISRDNAKNT VYLQMNSLKPEDTAVYYCNVKDAGEGQEQYDYWGQGT QVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
| GroEL2 | 33 | 16,356 | Monomeric | 244 | No | QVQLVESGGGLVQAGGSLRLSCAASGFPVYQHWMYWY RQAPGKEREWVAAIMSQGAGTWYADSVRGRFTISRDNA KNTVYLQMNSLKPEDTAVYYCNVKDLGKAEYNYDYWGQ GTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
a
NA, not applicable.