FIG 5.

Downregulated genes in the liver and spleen during peak RVFV infection. (A and B) Heat maps of sets of genes significantly downregulated after RVFV infection in liver (A) and spleen (B) tissues. Samples were clustered based on their infection status, as indicated on top of the heat maps. Genes were clustered into categories based on their molecular function or biological process, as indicated at the left of the heat maps. To represent the magnitude of the log₂ fold change of each gene compared to the mean gene expression level, cells are color-coded in shades of a gradient ranging from dark blue (low) to dark red (high). (C) Expression profiles of 6 selected genes significantly downregulated in RVFV-infected (Inf.) liver or spleen samples at 2 days post-infection (dpi) (group 2) and 4 days post-infection (group 3) compared to uninfected (Uninf.) samples (group 1). Dots represent individual normalized gene counts (on a log₁₀ scale), and the shaded area shows the distribution of the samples within each group. Abbreviations: VST, variance-stabilizing transformation; met., metabolism; CHO, carbohydrate metabolism; TF, transcription factor; IR, immune response; Struc., structural; Others, genes involved in folate metabolism, carotenoid metabolism, purine metabolism, signaling receptor activity, cell growth, hemostasis, and senescence.