Mean pre-treatment intensities of itch and pain. Participants were asked to rate itch and pain intensities on a numerical rating scale (NRS, 0–10). Mean intensities (with standard error of the mean; SEM) of itch and pain are presented separated by insect species and (A) sex of participants, and (B) age of participants. Colours from light green to dark red indicate milder to more severe ratings. **p < 0.01; ***p < 0.001, CLA = 0.57, (H0:μfemale = μfemale); CLB(itch) = 0.39, CLB(pain) = 0.41 (H0:μ19-25 = μ36-45 and H0:μ26-35 = μ56-65, respectively).