Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 29;220(9):e20221939. doi: 10.1084/jem.20221939

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Chancellor et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Self-reactivity and polyfunctionality of circulating MAIT cells from healthy donors. (A) CD137 expression by autoreactive MAIT cells expanded for 10 d. Proliferating and not proliferating MAIT (top row) or non-MAIT (bottom row) cells following stimulation with the indicated APCs ± anti-MR1 mAb (aMR1). MAIT cells (Vα7.2⁺/CD161⁺) proliferative status is revealed by CTV emission. Plots are representative of results obtained with six donors. (B) Summary of MAIT cell CD137 expression on proliferating cells (CTV dull) after rechallenge with the indicated condition (numbers as in panel A). Data were obtained from six donors. Statistical significance was determined using a one-way ANOVA with Friedman test, * P ≤ 0.05. (C) Effect of aMR1 mAb on surface expression of the indicated activation markers on CTV-dull MAIT cells stimulated with 5-OP-RU–pulsed THP-1 cells (top row) or with A375b-wtMR1 cells without exogenous antigens (bottom row). Median fluorescence intensity (MFI) is indicated ± aMR1 mAb. Data obtained from five donors. Statistical significance was determined using Student’s t test, * P ≤ 0.05, ** P ≤ 0.01. (D) Vα7.2 surface expression on MAIT cells stimulated with 5-OP-RU–pulsed THP-1 cells (top row) or with A375b-wtMR1 cells without exogenous antigens (bottom row). MFI is indicated ± aMR1 mAb. Data obtained from five donors. Statistical significance was determined using Student’s t test, * P ≤ 0.05. (E) Percentage of ex vivo MAIT cells from healthy donors double positive for CD137 and CD69 after overnight co-culture with A375b-MR1 cells ± aMR1 mAb. Stimulation with 5-OP-RU was used as positive control with the scale on the right-hand y-axis (green). Cells were pregated as CD3⁺/CD26⁺Vα7.2⁺/CD161⁺. Data are a summary of all five donors tested. Statistical significance was determined using Student’s t test, * P ≤ 0.05. (F and G) Average frequency of cells expressing one or more of the indicated activation-associated molecules within self-reactive MAIT cell lines stimulated with (F) A375b-wtMR1 cells or (G) 5-OP-RU–loaded THP-1 cells. Pie segments indicate cells positive for any combination of the indicated cytokines or activation markers. Pie arcs indicate the cytokine positivity of each segment. Data is averaged from five donors. Source data are available for this figure: SourceData F1.