Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 29;220(9):e20221939. doi: 10.1084/jem.20221939

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Chancellor et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Crossreactivity of circulating MAIT cells from healthy donors. (A) Percentage of CD137⁺ MAIT cells following activation by A375b-MR1 cells ± aMR1 mAb. MAIT cell lines were previously generated from two donors by in vitro expansion with 5-OP-RU. (B) Plots of MAIT cell lines stained with MR1-3-F-SA tetramer vs. CTV. Cells were pregated on CD161⁺ cells. Data were obtained from a total of two donors. (C) Representative plots of MAIT cell lines stained with MR1-6-FP tetramer vs. CTV. Cells were pre-gated on CD161⁺ cells. Data was obtained from a total of two donors. (D) Populations of MAIT cells that are double positive for MR1-3-F-SA and MR1-6-FP tetramers in the same two donors (B–D). (E) Frequency of MR1-5-OP-RU, -6-FP, -3-F-SA, or -5-F-SA MR1 tetramer⁺ cells from two additional MAIT cell lines derived from the peripheral blood of donors 3 and 4. Pie segments indicate cells positive for any combination of the four tetramer sets. Pie arcs indicate the tetramer positivity of each segment. Percentages indicate the total number of cells positive for at least one tetramer. (F) Percentage of ex vivo MAIT cells from healthy donors stained with at least one of three tetramers: CD8-null MR1-3-F-SA, -5-F-SA, or -6-FP. MAIT cells were pregated on live CD3⁺/Vα7.2⁺/CD161⁺/CD26⁺ cells. Non-MAIT cells were pregated on live CD3⁺/Vα7.2⁻ cells. Statistical significance was determined using Student’s t test, ** P ≤ 0.01. Source data are available for this figure: SourceData F3.