Figure 6.

Enrichment of R96 in self-reactive MAIT cells. (A) Frequencies of TRBV6⁺, CDR3L13⁺ MAIT cells with either R95, R96, R97, or R98 motif within either self-reactive, proliferated MAIT cells (CTV−) or non-self-reactive, non-proliferated MAIT cells (CTV+). Statistical significance was determined using Fisher’s exact test, * P ≤ 0.05. (B) Activation of J.RT3-T3.5 cells transduced with 393 TRBV (a MAIT TRBV bearing the E8-like motif) or with the control MRC25 TCRBV gene. Percentage of CD69⁺ cells after co-culture with the indicated APC ± anti-MR1 mAb is illustrated. 5-OP-RU–pulsed THP-1 cells were used as a positive control. Data are representative of three individual experiments each performed in triplicate. Statistical significance was determined using Student’s t test, *** P ≤ 0.001. (C) Frequency of TRBV sequences with the E8-like motif within ex vivo MAIT cells (TRAV1-2⁺/TRBV6⁺, and CD161⁺) or non-MAIT cells (TRAV1-2⁺/TRBV6⁺, and CD161⁻) sorted from the periphery of seven healthy donors (Lepore et al., 2014). Statistical significance was determined using Wilcoxon signed-rank test, * P ≤ 0.05.