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. 2023 Jun 29;220(9):e20221939. doi: 10.1084/jem.20221939

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© 2023 Chancellor et al.

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Figure S3. — Gating strategies for identifying bona fide MAIT cells. (A) Gating strategy for identifying MAIT (CD161+) and non-MAIT cells (CD161−) after enrichment for TCR Vα7.2+ T cells, linked to Fig. 3, B–D. (B) Example plots of CD161-fraction that does not contain many 5-OP-RU expanded T cells or T cells that bound to the MR1-5-OP-RU tetramer. (C) Example plots showing that CD161 hi cells within the line simultaneously proliferated when stimulated with 5-OP-RU and bound to the MR1-5-OP-RU tetramer. (D) Example of enrichment of MAIT cells with Vα7.2 mAb. Upper panel shows depleted fraction and lower panel shows enriched fraction. (E) Gating strategy for the identification of MAIT and non-MAIT cells displayed in Fig. 3 E. (F and G) Plots showing populations of MAITs or non-MAITs with different combinations of tetramers.