Skip to main content
. 2023 Jun 19;16(6):dmm049132. doi: 10.1242/dmm.049132

Fig. 6.

Fig. 6.

mESCs harbouring the OGTC921Y variant downregulate Sox2 and Oct4 in response to clonogenic conditions. (A) Schematic representation of the experimental set up. The experiments were performed using three cell clones per genotype and repeated over three passages. (B) Immunoblot showing detection of O-GlcNAcylation (RL2 antibody), OGT, OGA, Oct4 and Sox2 in mESC lines carrying OGTWT or the OGTC921Y variant. Lysates were derived from cells grown under clonogenic conditions in the presence of LIF as shown in A. (C-G) Quantification of OGT (C), RL2 (D), OGA (E), Sox2 (F) and Oct4 (G) immunoblot signals. OGT, RL2 and Sox2 signals were normalised to those of tubulin; OGA and Oct4 signals were normalised to those of actin. OGTWT, n=9 biological replicates (except for RL2, where n=8 biological replicates); OGTC921Y, n=9 biological replicates. Unpaired two-tailed t-test; P=0.0004 (C); P=0.067 (D); P=0.077 (E); P=0.0023 (F); P=0.0038 (G). Error bars represent s.d. AU, arbitrary units. NS, not significant; **P<0.01; ***P<0.001.