Fig. 4.

P. berghei RSP9 in asexual and sexual development. (A) Asexual growth of Δrsp9-gfp and wt-gfp recorded from day 4–6 until day 11 post infection. (B,C) Gametocytaemia (B) and gametocyte sex ratio (C) were determined by infections of three mice each infected with Δrsp9-gfp or wt-gfp. Error bars represent s.e.m. (D) Ookinete conversion rate of Δrsp9-gfp and wt-gfp as well as Δrsp9-gfp transfected with rescue or mock plasmid. Error bars represent s.e.m. ****P<0.0001, determined by Welch's t-test. (E) Δrsp9-gfp and wt-gfp gametocytes crossed with either Δcdpk4 (male deficient) or Δnek4 (female deficient) strains. Error bars represent s.e.m. **P<0.01, determined by Welch's t-test. (F) Anti-α-tubulin staining microtubules of exflagellating wt-gfp and Δrsp9-gfp male gametocytes (red) with nuclei stained with DAPI (arrows). Scale bars: 2 µm. (G) Number of Δrsp9-gfp oocysts compared to wt-gfp oocysts in mosquito midguts. Bars depict mean with s.e.m.