Table 1. Oligonucleotide primers used for RTqPCR and chromatin immunoprecipitation (ChIP) assay.
RTqPCR | |
Gene | Primer sequence (5’ → 3’) |
ccnb1.2 | Fwd: GAGGATGCACAAGCAGTCAG Rev: TTTGGGAACTGGGTGTTCCT |
ccna2 | Fwd: TTTGACCTTGCTGCTCCAAC Rev: CGCAGGAAAGGATCAGCATC |
cdk1 | Fwd: GGAACGCCCAACAATGAAGT Rev: CAGGTCCAGCCCATCCTTAT |
e2f1 | Fwd: CGCTGACGTTGTGTATGGTT Rev: TTCCTGTAGGCATTCACGGT |
a-actinin (reference gene) | Fwd: GGACAATTATCCTGCGTTTTGC Rev: CCTTCTTTGGCAGATGTTTCTTC |
ChIP assay | |
Genomic region | Primer sequence (5’ → 3’) |
thrb TRE | Fwd: CCCCTATCCTTGTTCGTCCTC Rev: GCGCTGGGCTGTCCT |
klf9 synergy module (KSM) | Fwd: CCGTCCCTTCTTTTGTGTACATT Rev: GCTGTTCGTGCCACTTTGC |
thibz TRE | Fwd: GGACGCACTAGGGTTAAGTAAGG Rev: TCTCCCAACCCTACAGAGTTCAA |
ifabp promoter | Fwd: CCCTACATTGGTTGAGCCAGTTTT Rev: TCAAAGGCCATGGTGATTGGT |
thrb exon 5 | Fwd: CCCCGAAAGTGAAACTCTAACTCT Rev: CCACACCGAGTCCTCCATTTT |