Skip to main content
. 2023 Jun 29;18(6):e0287858. doi: 10.1371/journal.pone.0287858

Table 1. Oligonucleotide primers used for RTqPCR and chromatin immunoprecipitation (ChIP) assay.

RTqPCR
Gene Primer sequence (5’ → 3’)
ccnb1.2 Fwd: GAGGATGCACAAGCAGTCAG
Rev: TTTGGGAACTGGGTGTTCCT
ccna2 Fwd: TTTGACCTTGCTGCTCCAAC
Rev: CGCAGGAAAGGATCAGCATC
cdk1 Fwd: GGAACGCCCAACAATGAAGT
Rev: CAGGTCCAGCCCATCCTTAT
e2f1 Fwd: CGCTGACGTTGTGTATGGTT
Rev: TTCCTGTAGGCATTCACGGT
a-actinin (reference gene) Fwd: GGACAATTATCCTGCGTTTTGC
Rev: CCTTCTTTGGCAGATGTTTCTTC
ChIP assay
Genomic region Primer sequence (5’ → 3’)
thrb TRE Fwd: CCCCTATCCTTGTTCGTCCTC
Rev: GCGCTGGGCTGTCCT
klf9 synergy module (KSM) Fwd: CCGTCCCTTCTTTTGTGTACATT
Rev: GCTGTTCGTGCCACTTTGC
thibz TRE Fwd: GGACGCACTAGGGTTAAGTAAGG
Rev: TCTCCCAACCCTACAGAGTTCAA
ifabp promoter Fwd: CCCTACATTGGTTGAGCCAGTTTT
Rev: TCAAAGGCCATGGTGATTGGT
thrb exon 5 Fwd: CCCCGAAAGTGAAACTCTAACTCT
Rev: CCACACCGAGTCCTCCATTTT