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NR supplementation strategy to bypass low cardiac Nampt levels. (A) Relative Nampt and (B) Nmrk2 mRNA expression in hearts from 2-month-old female αMHC-Cre+ Rev-erbα/β double floxed (CM-RevDKO) vs littermate controls (αMHC-Cre- Rev-erbα/β double floxed) (n = 3 hearts/genotype/timepoint; ***p < 0.001 by two-way ANOVA). (C) Schematic representation of NAD+ boosting strategy in CM-RevDKO mice with NR.
