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. 2023 Jun 29;13:10574. doi: 10.1038/s41598-023-37354-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Window of susceptibility (yellow) laid upon the curve of the average weight development of all animals of the Placebo group (A) Randomization prior application led to no significant average weight differences (B). The outcome after the application of 70 µg biliatresone depends on the injection weight. Animals in the treatment group with the outcome of no signs of cholestasis had an average weight of 1.64 g (n = 7). The weight of animals developing clinical signs of cholestasis had an average weight of 1.60 g (n = 8). An average injection weight of 1.44 g was observed in pups that died within 2 days after the injection (n = 4) (C).