Fig. 3.

Vitamin B6 attenuates type 2 inflammation via IL-33. a Q-PCR was used to detect the expression of IL-5 and IL-13 in lungs from PBS-treated mice or PLP-treated papain model mice (n = 6). b Q-PCR was used to detect the expression of IL-25, IL-33, and TSLP in the lungs from the PBS-treated mice or the papain-treated papain model (n = 4/6). c, d Representative flow cytometry analysis and quantification of eosinophils in BALF in an anti-IL-33 antibody-treated papain model. e ELISA was used to determine the amounts of IL-5 and IL-13 in BALF in an anti-IL-33 antibody-treated papain model. f Representative H&E and PAS staining of lung sections (scale bar, 100 µm). Inflammation was determined by semiquantitative scoring. The data are shown as the means ± SDs (error bars) and represent three independent experiments. The data were analyzed by unpaired Student’s t test (a, b) or ordinary one-way ANOVA with Sidak’s test (c–f). Ns not significant. *p < 0.05; **p < 0.01; ****p < 0.0001