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. 2023 May 23;20(7):794–807. doi: 10.1038/s41423-023-01029-6

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Fig. 4 — Vitamin B6-regulated IL-33 homeostasis is dependent on PDXK. a Schematic diagram of PBS or PLP treatment by the intratracheal route for 3 days. BALF and lung homogenate were collected 1 h after challenge with papain at Day 4. b The amount of IL-33 in BALF was determined by ELISA (n = 4). The results of one of three experiments are shown. Unpaired Student’s t test was used to analyze the data. *p < 0.05. c The lung tissue of mice was weighted and addded 2.5 mL/g PBS to homogenize, and then the amounts of IL-33 in the supernatant of lung homogenates were determined by ELISA (n = 4). The results of one of three experiments is are. Unpaired Student’s t test was used to analyze the data. *p < 0.05. d, e Western blot detection of the protein level of IL-33 in A549-IL-33 cells treated with 0.5 mM PL for the duration indicated. e The ratio of HA-IL-33/actin is shown (n = 3). The results for one of three experiments are shown. Ordinary one-way ANOVA with Dunnett’s multiple comparisons test was used to analyze the data. ****p < 0.0001. f A549-IL-33 cells or PDXK-knockout A549-IL-33 cells were treated with the indicated concentrations of PL for 24 h, and western blotting was used to analyze intracellular IL-33 protein levels. The data are representative of three independent experiments. g The ratio of HA-IL-33/actin in (a) is shown (n = 3). Ordinary one-way ANOVA with Dunnett’s multiple comparisons test was used to analyze the data. ***p < 0.001; ns not significant. h HEK293FT cells were transfected with HA-IL-33 or His-PDXK for 24 h, treated with or without 0.5 mM PL for 24 h, and analyzed by western blotting. The results of one of three experiments are shown. i The HA-IL-33/actin ratio in (c) is shown (n = 3). Ordinary one-way ANOVA with Tukey’s multiple comparisons test was used to analyze the data. *p < 0.05; **p < 0.01. j PLP levels in the serum of PDXK^+/+ (n = 6) and PDXK^+/− (n = 4) mice according to LC‒MS. Unpaired Student’s t test was used to analyze the data. *p < 0.05. The data are representative of three independent experiments. k ELISA was used to detect the IL-33 levels in the supernatant of lung homogenates of PDXK^+/+ (n = 7) and PDXK^+/− (n = 6) mice. Unpaired Student’s test was used to analyze the data. **p < 0.01. The data are representative of two independent experiments