Fig. 9. Differential expression of HIF-1α in liver macrophages of ALF in WT and CB2R^−/− mice.

Liver sections of mice were treated with F4/80⁺ and HIF-1α immunofluorescence co-staining 6 h after indicated treatment (×400) in a WT mice and b CB2R^−/− mice. c Quantitative analysis of F4/80⁺ expression by Image J. d Analysis of liver macrophage with HIF-1α staining positive. (n = 4, 6, 5, 5, 4, 5, 4, respectively). (ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001)