Figure 3.

IRG1/4-OI inhibited inflammatory response. (A–C) The serum levels of IL-1β, TNF-α, and IL-6 were detected by ELISA. (D–F) The expressions of IL-1β, TNF-α, and IL-6 mRNA of colon tissue were detected by RT-PCR. (G–J) The expression of p-p65 in the IRG1^−/− group was significantly increased, and the expression of Nrf2/HO-1 protein was significantly reduced compared with the WT group, and these changes were significantly reduced after IRG1^−/− mice received 4-OI treatment. Data are shown as the mean ± SD, ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.