Fig. 2.

Screening of drug libraries for compounds that inhibit p72-Fluc reporter. A Flow chart of high throughput screening. HEK293T cells were transfected with plasmids of p72-Fluc system and seeded in 96 well plates, afterwards, 3200 compounds were added into the plate by liquid handler, the luciferase intensity were read out by a microplate reader. B Standard scores analysis of high throughput screening results. The fluorescence signal value was normalized to Z-scores defined by (F − F_mean)/F_sd, those candidates with values less than −3 were selected as potential hits. The intersection of the hits from the duplicate experiments is displayed by a Venn diagram (C).