Fig. 6.

Downregulation of p23 inhibited the ASFV replication. A HEK293T cells were treated with si-p23 or AIL, and then transfected with p72-Fluc system. The luminescence of p72-Fluc were acquired by a multiplate reader. B HEK293T cells were transfected with p23 si-RNA or treated with AIL, and then transfected with the ASFV polymerase subunits H359L, C147L or GFP as control. H359L, C147L or GFP were detected by Western blotting. NTC indicated nontargeting control siRNA. C PAMs cells were treated with si-p23 or AIL, and then infected with ASFV-eGFP (MOI ​= ​0.1), the relative expression of ASFV-p72 were measured by RT-qPCR at day 2 post-infection. Statistical significance is denoted by ∗∗∗P ​< ​0.001.