Fig. 7.

Ailanthone inhibits the replication of Zika virus in vitro.A The inhibitory effect of AIL against Zika virus and cytotoxicity of AIL in Vero cells were analyzed by RT-qPCR and CellTiter-Glo. B Vero cells were infected with Zika virus (MOI ​= ​0.7), and treated with different concentrations of AIL. The fluorescence images of the viral infections were captured by a microscope. C The relative infection rate of Zika virus was calculated by the Harmony high-content imaging and analysis software (Perkinelmer, USA, Opera phenix) according to the manufacturer's instruction. Scale bar indicated 75 ​μm. Statistical significance is denoted by ∗ P ​< ​0.05, and ∗∗P ​< ​0.01.