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. Author manuscript; available in PMC: 2023 Jun 30.
Published in final edited form as: Cell Host Microbe. 2023 Jun 5;31(6):1038–1053.e10. doi: 10.1016/j.chom.2023.05.011

Figure 4. Gut bacterial isolates use purines as carbon and energy sources.

Figure 4.

Anaerobic growth of bacterial strains on plates containing soluble (glucose, allantoin), and insoluble (uric acid and adenine) substrates. As detailed in Methods, plates were inoculated with 4 μl of dense overnight cultures grown in rich medium then incubated for 2 days [no fermentable substrate, glucose, allantoin or uric acid conditions] or 7 days (adenine). Growth is indicated by the appearance of cell patches and a zone of clearing for the overlay plates. Details about strains are specified in the Key Resources Table, and a summary of all tested strains is presented in Supplemental Figure 4. Strains indicated in red were used for colonization of gnotobiotic mice.