Figure 1: In vitro physiological inhibition of PAR2 activation by C781.

Traces show PAR2 activation and subsequent inhibition in 16HBE14o- cells by (A) 2-at-LIGRL-NH₂ (2AT); (B) Trypsin; and (C) Elastase as measured by xCELLigence RTCA. Micromolar concentrations of C781 effectively reduce the in vitro physiological response to both ligand and proteinase agonists. Each trace represents the average of n = 4 (2AT, trypsin) or n = 3 (elastase) experiments from a single E-plate and are representative of multiple E-plates (2AT, E-plate n = 6; trypsin, n = 3; elastase, n = 2). Error bars are eliminated for clarity. Activating compound concentrations were EC₈₀ and determined in (Rivas et al., 2022).