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(2S)-Eriodictyol production in E. coli strains containing flavonoid 3′-hydroxylase (F3′H) from different species and P450 reductase (CPR) from A. thaliana or CPR mutants. (a) Construction of plasmids harboring F3′H and CPR. pBR322: pETDuet-1; arrow: T7 promoter; rectangle: F3′H genes from different species, and CPR or trCPR gene. (b) Synthesis of (2S)-eriodictyol from strains engineered with various F3′Hs and CPR mutants supplemented with (2S)-naringenin (100 mg L–1).
